Name your design:  

Please enter the sequence or Accession number to design a siRNA target sequence:

  Sequence (raw or fasta)     Accession number  

 Please choose the range of GC content percentage:

 %GC Values between 10 20 30 40 50 60 70 80 90 and 10 20 30 40 50 60 70 80 90 100

 Avoid 4 or more  nucleotide  runs: Yes       No

 Choose ORF:Yes       No

 Choose your pattern for siRNA target design(target sequence 5' - 3'):         

  A N R Y T G C A N R Y T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C N R Y A T G C T N R Y A G C T N R Y A G C

 Where   N -- Any nucleotide     Y-- Pyrimidine (C or T)        R -- Purine (A or G)         A -- Adenine      G -- Guanine       C -- Cytosine       T -- Thymine

 Choose a preferred blast method:NCBI BLAST       WU BLAST

  Apply scoring system